Analysis Note

ControlMouse heart lysate

Application

Detect Doublecortin using this mouse monoclonal antibody, Anti-DCX Antibody, clone 2G5 validated for use in western blotting, IHC, ICC & Flow Cytometry.

Research CategoryNeuroscience

Research Sub CategoryDevelopmental Neuroscience

Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected DCX in human brain and kidney cancer tissues.

Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected DCX in HepG2 cells.

Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected DCX in non serum starved SK-N-SH cells.

Optimal working dilutions must be determined by end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Doublecortin (DCX) or alternatively known as Doublin or Lissencephalin-X (Lis-X) and encoded by the human gene named DCX/DBCN/LISX is a microtubule associated protein that is required for the initial steps of neuronal dispersion and lamination during cortex brain development. Expressed by neuronal precursor cells and immature neurons, DCX expression is decreased by the time neurons are mature and express the mature neuronal marker NeuN. Thus DCX expression is a hallmark of developing neurons and neurogenesis. DCX expression is localized in the cytoplasm and along microtubule tracks. DCX appears to stabilize microtubules and causes bundling of them in the cytoplasm. In disease, DCX is the cause for an X-linked lissencephaly and subcortical laminar heterotopia, a disorder that is characterized by a “smooth” brain with few folds like normal brains and poorly formed cortical layers as well as other abnormalities. Besides developmental effects DCX may potentially help in the suppression of gliomas because in brain tumor stem cells (BTSC) self-renewal ability was significantly reduced in cells expressing DCX compared to control cells. EMD-Millipore's Anti-DCX mouse monoclonal has been tested in western blot against recombinant protein as well as mouse heart lysate. The antibody has also been successfully tested in paraffin embedded immunohistochemistry on normal human brain and kidney cancer tissue sections as well as in fluorescent immunocytochemistry on HepG2 cells in culture and flow cytometry on SK-N-SH cells in culture and direct ELISA on varying concentrations of antigen.

Immunogen

Purified recombinant fragment of human DCX expressed in E. Coli.

Physical form

Protein G Purified

Purified mouse monoclonal in PBS with up to 0.1% sodium azide and 0.5% protein stabilizer.

Format: Purified

Quality

Evaluated by Western Blotting in Mouse heart lysate.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected DCX in mouse heart lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~50 kDa observed. Uncharacterized bands may appear in some lysate(s).